Distribution of sulphur, phosphorous, iron, and trace elements in bottom sediment cores, mussels and fish from the Punat Bay (Island of Krk, Croatia)

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Tatjana Ivošević
Ivan Halkijević
Nina Bilandžić
Katarina Licht
Marija Sedak
Ivica Orlić
Damir Bucković
Štefica Kampić


Ships and associated anthropogenic activities release a number of contaminant elements into the marine  environment which can be particularly concentrated in restricted circulatory environments including Bays and marinas. One such locality is a Punat Bay, situated at the southern coastline of the island of Krk (west Croatia), which is the largest Croatian marina. The aim of this study was to examine depth profiles of sulfur (S), phosporous (P), iron (Fe), and trace elements (As, Ba, Be, Cd, Co, Cr, Cu, Hg, Mn, Mo, Ni, Pb, Sn, Sr, Ti, U, V, Y, and Zn) in six sediment cores (down to 20-30 cm), and from mussels and fish from Punat Bay, by determining their levels with ICP-OES. Data analysis showed that the majority of variables were elevated in a sediment core located closest a port of the marina. Minimum to maximum S, P, and Fe levels in sediments were as follows: 0.4-2.4%, 0.04-320 mg/kg, and 0.2-2.3%, respectively. Correlations among S, P, Fe, and various trace elements were mostly positive (p<0.05). Trace elements were not increased in mussels and fish. This paper shows that the Punat marina has only a limited impact on the environmental status. Several potentially toxic trace elemens (Pb, Cu, etc.) were found to be elevated in a sediment core located closest to the marina.


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ABDOLAHPUR MONIKH, F., HOSSEINI, F., PERRY, S., MARYAMABADI, A., GHANEMI, K., BASTAMI, A.A. & KARAMI, O. (2011): Heavy metals levels in sediment and Ray fish (Dasyatis bennettii) from Musa estuary, Persian Gulf.- American-Eurasian Journal of Toxicological Sciences, 3/4, 224–227.

AGAH, H., LEERMAKERS, M., ELSKENS, M., FATEMI, S.M. & BAEYENS, W. (2009): Accumulation of trace metals in the muscle and liver tissues of five species from the Persian Gulf.– Environmental Monitoring Assessment, 157, 499–514. doi: 10.1007/s10661-008-0551-8

BAJC, Z. & KIRBIŠ, A. (2019): Trace Element Concentrations in Mussels (Mytilus galloprovincialis) from the Gulf of Trieste, Slovenia.– Journal of Food Protection, 82/3, 429–434. doi: 10.4315/0362-028X.JFP-18-378

BOGDANOVIĆ, T., UJEVIĆ, I., SEDAK, M., LISTEŠ, E., ŠIMAT, V., PETRIČEVIĆ, S. & POLJAK, V. (2014): As, Cd, Hg and Pb in four edible shellfish species from breeding and harvesting areas along the eastern Adriatic Coast, Croatia.– Food Chemistry, 146, 197–203. doi: 10.1016/j.foodchem.2013.09.045

BRUNOVIĆ, D., MIKO, S., ILIJANIĆ, N., PEH, Z., HASAN, O., KOLAR, T., ŠPARICA MIKO, M. & RAZUM, I. (2019): Holocene foraminiferal and geochemical records in the coastal karst dolines of Cres Island, Croatia.– Geologia Croatica, 72/1, 19–42. doi: 10.4154/gc.2019.02

BUSKEY, E.J., WHITE, H.K. & ESBAUGH, A.J. (2016): Impact of oil spills on marine life in the Gulf of Mexico: Effect on plankton, nekton, and deep-sea benthos.– Oceanography, 29, 174–181. doi: 10.5670/oceanog.2016.81

CARDELLICCHIO, N., BUCCOLIERI, A., DI LEO, A., GIANDOMENICO, S. & SPADA, L. (2008): Levels of metals in reared mussels from Taranto Gulf (Ionian Sea, Southern Italy).– Food Chem., 107, 890–896. doi: 10.1016/j.foodchem.2007.09.011

CINDRIĆ, A.M., GARNIER, C., OURSEL, B., PIŽETA, I. & OMANOVIĆ, D. (2015): Evidencing the natural and anthropogenic processes controlling trace metals dynamic in a highly stratified estuary: The Krka River estuary (Adriatic, Croatia).– Mar. Pollut. Bull., 94, 199–216. doi: 10.1016/j.marpolbul.2015.02.029

COLINA M., GARDINER, P., RIVAS, Z. & TRONCONE, F. (2005): Determination of vanadium species in sediment, mussel and fish muscle tissue samples by liquid chromatography-inductively coupled plasma-mass spectrometry.– Anal. Chim. Acta, 538, 107–115. doi: 10.1016/j.aca.2005.02.044

CUKROV, N., FRANČIŠKOVIĆ-BILINKSI, S., HLAČA, B. & BARIŠIĆ, D. (2011): A recent history of metal accumulation in the sediments of Rijeka harbour, Adriatic Sea, Croatia.– Mar. Pollut. Bull. 2011, 62, 154–167.

ĆOSOVIĆ, V., MARJANAC, T., DROBNE, K. & MORO, A. (2008): Outer Dinarides: eastern Adriatic coast. Paleogene and Neogene.– In: MCCANN, T. (ed.): The Geology of Central Europe, Volume 2: Mesozoic and Cenozoic. The Geological Society London, 1031–1139.

EUROPEAN COMMISSION REGULATION (EC) NO. 1881/2006 setting maximum levels for certain contaminants in foodstuffs. Available online: http://extwprlegs1.fao.org/docs/pdf/eur68134original.pdf

FARD, N.J.H., RAVANBAAKHSH, M., RAMEZANI, Z., AHMADI, M., ANGALI, K.A. & JAVID, A.Z. (2015) Determination of mercury and vanadium concentration in Johnius belangerii (C) fish in Musa estuary in Persian Gulf.– Marine Pollution Bulletin, 97/1–2, 499–505. doi: 10.1016/j.marpolbul.2015.05.036

FIKET, Ž., MIKAC, N. & KNIEWALD, G. (2017a): Sedimentary records of the Zrmanja River Estuary, eastern Adriatic coast—Natural vs. anthropogenic impacts.– J. Soils Sediments, 17, 1905–1916. doi: 10.1007/s11368-016-1504-4

FIKET, Ž., MIKAC, N. & KNIEWLAD, G. (2017b): Mass Fractions of Forty-Six Major and Trace Elements, Including Rare Earth Elements, in Sediment and Soil Reference Materials Used in Environmental Studies.– Geostand. Geoanalytical. Res., 41, 123–135. Doi: 10.1111/ggr.12129

FIKET, Ž., FIKET, T., IVANIĆ, M., MIKAC, N. & KNIEWALD, G. (2019): Pore water geochemistry and diagenesis of estuary sediments—An example of the Zrmanja River estuary (Adriatic coast, Croatia).– J. Soils Sediments, 19, 2048–2060. doi: 10.1007/s11368-018-2179-9

FIKET, Ž., PETROVIĆ, M., MEDUNIĆ, G., IVOŠEVIĆ, T., FIKET, T., ZHANG XU, L., WANG, Y. & DING, S. (2021): Evaluation of the potential release tendency of metals and metalloids from the estuarine sediments: case study of Raša Bay.– Molecules, 26, 6656. doi: 10.3390/molecules26216656

FOX, M., MITCHELL, M., DEAN, M., ELLIOTT, C. & CAMPBELL, K. (2018): The Seafood Supply Chain from a Fraudulent Perspective.– Food Security, 10, 939–963. doi: 10.1007/s12571-018-0826-z

FURDEK TURK, M., IVANIĆ, M., DAUTOVIĆ, J., BAČIĆ, N & MIKAC, N. (2020): Simultaneous analysis of butyltins and total tin in sediments as a tool for the assessment of tributyl behaviour, long-term persistence and historical contamination in the coastal environment.– Chemosphere, 127307. doi: 10.1016/j.chemosphere.2020.127307

GOMES, T., PINHEIRO, J. P., CANCIO ,I., PEREIRA, C.G., CARDOSO, C. & BEBIANNO, M. (2011): Effects of copper nanoparticles exposure in the mussel Mytilus galloprovincialis.– Environmental Science and Technology, 45/21, 9356–9362. doi: 10.1021/es200955s

GULAM, V., POLLAK, D. & PODOLSZKI, L. (2014): The analysis of the ¬ flysch badlands inventory in central Istria, Croatia.– Geologia Croatica, 67/1, 1–15. doi: 10.4154/GC.2014.01

HAMMER, Ø., HARPER, D.A.T. & RYAN, P.D. (2001): PAST: Paleontological statistics software package for education and data analysis.– Palaeontol. Electron. 4, 1–9. Available online: http://palaeo482electronica.org/2001_1/past/issue1_01.htm (accessed on June 10, 2022).

HAN, M., ZHANG, J., CHU, W., CHEN, J. & ZHOU, G. (2019): Research progress and prospects of marine oily wastewater treatment: a review.– Water, 11/12, 2517. doi: 10.3390/w11122517

JEBREEN, H., WOHNLICH, S., BANNING, A., WISOTZKY, F., NIEDERMAYR , A. & GHANEM, M. (2018): Recharge, geochemical processes and water quality in karst aquifers: Central West Bank, Palestine.– Environmental Earth Sciences, 77/6, 261. doi: 10.1007/s12665-018-7440-4

KANG, S., IVOŠEVIĆ, T., MEDUNIĆ, G. & DAI, S. (2022): Coal-derived sulphur and selenium in marine sediment cores (Raša Bay, Croatia): recommended steps of analysing environmental earth data.– In: MALVIĆ, T. & IVŠINOVIĆ, J. (eds.): Mathematical methods and terminology in geology. Zagreb, Rudarsko-geološko-naftni fakultet, 75–83.

KLJAKOVIĆ-GAŠPIĆ, Z., ZVONARIĆ, T., VRGOČ, N., ODŽAK, N. & BARIĆ, A. (2002): Cadmium and lead in selected tissues of two commercially important fish samples from the Adriatic Sea.– Water Research, 36, 5023–5028.

KLJAKOVIĆ-GAŠPIĆ, Z., OZAK, N., UJEVIĆ, I., ZVONARIĆ, T. & BARIĆ, A. (2006): Biomonitoring of trace elements (Cu, Cd, Cr, Hg, Pb, Zn) in eastern Adriatic using the Mediterranean blue mussels (2001-2005).– Fresenius Environ. Bull., 15/9a, 1041–1048.

KLJAKOVIĆ-GAŠPIĆ, Z., UJEVIC, I., ZVONARIC, T. & BARIC, A. (2007): Biomonitoirng of trace elements (Cu, Cd, Cr, Hg, Pb, Zn) in Mali Ston Bay (eastern Adriatic) using the Mediterranean blue mussel (1998-2005).– Acta Adriat., 48/1, 73–88.

LACERDA, L.D., MALM, O., GUIMARAES, J.R.D., SALOMONS, W. & WILKEN, R.-D. (1995): Mercury and the New Gold Rush in the South.– In: SALOMONS, W. & STIGLIANI, W.M. (eds.): Biogeodynamics of pollutants in soil and sediments. Risk assessment of delayed and non-linear responses. Springer, Berlin Heidelberg New York, 213–245.

MARIA, V.L. & BEBIANNO, M.J. (2011): Antioxidant and lipid peroxidation responses in Mytilus galloprovincialis, exposed to mixtures of benzo(a)pyrene and copper.– Comp. Biochem. Pharmacol. C, 154/1, 56–63. doi: 10.1016/j.cbpc.2011.02.004

MARKOVIĆ, J., JOKSIMOVIĆ, D. & STANKOVIĆ, S. (2012) Trace element concentrations in wild mussels from the coastal area of the southeastern Adriatic, Montenegro.– Arch. Biol. Sci, Belgrade, 64/1, 265–275. doi: 10.2298/ABS1201265M

MEDUNIĆ, G., KUHARIĆ, Ž., FIKET, Ž., BAJRAMOVIĆ, M., SINGH, A.L., KRIVOHLAVEK, A., KNIEWALD, G. & DUJMOVIĆ, L. (2018): Selenium and other potentially toxic elements in vegetables and tissues of three non-migratory birds exposed to soil, water, and aquatic sediment contaminated with seleniferous Raša coal.– Rudarsko-geološko-naftni zbornik, 33/3, 53–62.

MIHELČIĆ, G., ŠURIJA, B., JURAČIĆ, M., BARIŠIĆ, D. & BRANICA, M. (1996): History of the accumulation of trace metals in sediments of the saline Rogoznica Lake (Croatia).– Science of the Total Environment, 182, 105–115. doi: 10.1016/0048-9697(95)05058-2

MIKULIĆ, N., OREŠČANIN, V., LEGOVIĆ, T. & ZUGAJ, R. (2004). Estimation of heavy metals (Cu, Zn and Pb) input into Punat Bay.– Environmental geology, 46, 62–70. doi: 10.1007/s00254-004-1015-2

MILLER, J.R., LECHLER, P.J. & DESILETS, M. (1998): The role of geomorphic processes in the transport and fate of mercury in the Carson River basin, west-central Nevada.– Environmental Geology, 33, 249–262. doi: 10.1007/s002540050244

MU, Z., CHENG, Y., HUANG, Q., HU, M., DONG, W., FAN, J. & WANG, Y. (2021): Synchronous cycle of available phosphorus, iron, and sulfur in the sediment of Lancang River Reservoirs.– Water, 13, 2691. doi: 10.3390/w13192691

NOWROUZI, M., POURKHABBAZ, A. & REZAEI, M. (2012): Bioaccumulation and Distribution of Metals in Sediment sand Avicenna marina Tissues in the Hara Biosphere Reserve, Iran.– Bull. Environ. Contam. Toxicol., 89, 799–804. doi: 10.1007/s00128-012-0751-3

OREŠČANIN, V., MIKULIĆ, N., OBHOĐAŠ, J., NAĐ, K. & VALKOVIĆ, V. (2002): Distribution of trace elements in the coastal sea sediments: Punat Bay in the northern Adriatic.– Journal of Trace and Microprobe Techniques, 20/2, 247–260. doi: 10.1081/TMA-120003727

OREŠČANIN, V., BARIŠIĆ, D., LOVRENČIĆ, I., MIKELIĆ, L., ROŽMARIĆ- MAČEFAT, M., PAVLOVIĆ, G. & LULIĆ, S. (2005a): The influence of fly and bottom ash deposition on the quality of Kaštela Bay sediments.– Environmental Geology, 49, 53–64. doi: 10.1007/s00254-005-0058-3

OREŠČANIN, V., LULIĆ, S., MEDUNIĆ, G. & MIKELIĆ, L. (2005b): Granulometric and chemical composition of the Danube River sediments, Batina Village, Croatia.– Geologia Croatica, 58/2, 185–194.

PARISE, M., DE WAELE, J. & GUTIERREZ, F. (2009): Current perspectives on the environmental impacts and hazards in karst.– Environmental Geology, 58, 235–237. doi: 10.1007/s00254-008-1608-2

PARISE, M., CLOSSON, D., GUTIERREZ, F. & STEVANOVIĆ, Z. (2015): Anticipating and managing engineering problems in the complex karst environment.– Environmental Earth Sciences 74, 7823. doi: 10.1007/s12665-015-4647-5

PAVLOVIĆ, G., BARIŠIĆ, D., LOVRENČIĆ, I., OREŠČANIN, V. & PROHIĆ, E. (2005): Use of fallout 137Cs for documenting the chronology of overbank sediments from the river Sava, Croatia, and interpreting their geochemical patterns.– Environmental Geology, 47, 475–481. doi: 10.1007/s00254-004-1167-0

PAVLOVIĆ, G., PROHIĆ, E. & TIBLJAŠ, D. (2004): Statistical assessment of geochemical pattern in overbank sediments of the river Sava, Croatia.– Environmental Geology, 46, 132–143. doi: 10.1007/s00254-004-1019-y

PEROŠEVIĆ, A., JOKSIMOVIĆ, D., ĐUROVIĆ, D., MILAŠEVIĆ, I., RADOMIROVIĆ, M. & STANKOVIĆ, S. (2018): Human exposure to trace elements via consumption of mussels Mytilus galloprovincialis from Boka Kotorska Bay, Montenegro.– Journal of Trace Elements in Medicine and Biology, 50, 554–559. doi: 10.1016/j.jtemb.2018.03.018

POTTS, P.J. (1992). A handbook of silicate rock analysis.– Springer Science + Business Media, New York, 631 p. doi: 10.1007/978-1-4615-3270-5

RAĐENOVIĆ, A. & MEDUNIĆ, G. (2015): Adsorptive removal of Cr(VI) from aqueous solution by carbon black.– Journal of Chemical Technology and Metallurgy, 50/1, 81–88.

REIMANN, C. & DE CARITAT, P. (1998): Chemical elements in the environment. Factsheets for the geochemist and environmental scientist.– Springer-Verlag Berlin Heidelberg, Germany, 398 p.

REIMANN, C. & FILZMOSER, P. (2000): Normal and lognormal data distribution in geochemistry: death of a myth. Consequences for the statistical treatment of geochemical and environmental data.– Environmental Geology, 39, 1001–1014. doi: 10.1007/s002549900081

ROSLI, M., SAMAT, S., YASIR, M. & YUSOF, M. (2018): Analysis of heavy metal accumulation in fish at Terengganu coastal area, Malaysia.– Sains Malaysiana, 47/6, 1277–1283. doi: 10.17576/jsm-2018-4706-24

ŞAHIN, V. & VARDAR, N. (2020): Determination of wastewater behavior of large passenger ships based on their main parameters in the pre-design stage.– Journal of Marine Science and Engineering, 8, 8, 546.

SULIMANEC GRGEC, A., JURASOVIĆ, J., KLJAKOVIĆ-GAŠPIĆ, Z., ORCT, T., RUMORA SAMARIN, I., JANČI, T., SEKOVANIĆ, A., GRZUNOV LETINIĆ, J., MATEK SARIĆ, M., BENUTIĆ, A., CAPAK, K. & PIASEK, M. (2022): Potential risks and health benefits in the diet during the childbearing period: focus on trace elements and n-3 fatty acid content in commonly consumed fish species from the Adriatic Sea.– Environmental Advances, 8, 100226. doi: 10.1016/j.envadv.2022.100226

SALOMONS, W. & FOERSTNER, U. (1984): Metals in the hydrocycle.– Springer, Berlin Heidelberg New York, 349 p. doi: 10.1007/978-3-642-69325-0

SASMAZ, M., OBEK, E. & SASMAZ, A. (2019): Bioaccumulation of cadmium and thallium in Pb-Zn tailing waste water by Lemna minor and Lemna gibba.– Applied Geochemistry, 100, 287–292. doi: 10.1016/j.apgeochem.2018.12.011

SHU, Y., WANG, X., HUANG, Z., SONG, L., FEI, Z., GAN, L., XU, Y. & YIN, J. (2022): Estimating spatiotemporal distribution of wastewater generated by ships in coastal areas.- Ocean & Coastal Management, 222, 106133. doi: 10.1016/j.ocecoaman.2022.106133

SENESI, G.S., BALDASSARRE, G., SENESI, N. & RADINA, B. (1999): Trace element inputs into soils by anthropogenic activities and implications for human health.– Chemosphere. 39/2, 343–77. doi: 10.1016/s0045-6535(99)00115-0

STANKOVIĆ, S., TANASKOVSKI, B., ZLATIĆ, B., ARSENOVIĆ, M. & PEZO, L. (2014): Analysis of trace elements in surface sediments, mussels, seagrass and seawater along the southeastern Adriatic coast – a chemometric approach.– Pure and Applied Chemistry, 86/7, 1111–1127. doi: 10.1515/pac-2014-0201

ŠUŠNJAR, M., BUKOVAC, J., NIKLER, L., CRNOLATAC, I., MILAN, A., ŠIKIĆ, D., GRIMANI, I., VULIĆ, Ž. & BLAŠKOVIĆ, I. (1970): Osnovna geološka karta SFRJ 1:100 000, list Crikvenica [Basic Geological Map of SFRY 1:10000, Crikvenica sheet – in Croatian].– Institut za geološka istraživanja, Zagreb, Savezni geološki zavod, Beograd.

TERNJEJ, I., GAURINA SRČEK, V., MIHALJEVIĆ, Z. & KOPJAR, N. (2013): Cytotoxic and genotoxic effects of water and sediment samples from gypsum mining area in channel catfish ovary (CCO) cells.- Ecotoxicology and Environmental Safety 98, 119–127. doi: 10.1016/j.ecoenv.2013.09.014

VALKOVIĆ, O. & BOGDANOVIĆ, I. (1996): PIXE and XRF analysis of marine sediments.– Nuclear instruments and Methods in Physics Research B, 109/110, 488–492. doi: 10.1016/0168-583X(95)00956-6

VLAHOVIĆ, I., TIŠLJAR, J., VELIĆ, I. & MATIČEC, D. (2005): Evolution of the Adriatic carbonate platform; paleogeography, main events, and depositional dynamics.– Palaeogeography Palaeoclimatology Palaeoecology, 220, 333–360.

VLAHOVIĆ, I., TIŠLJAR, J., VELIĆ, I. & MATIČEC, D. (2007): Immense Tertiary Carbonate Jelar Breccia, Dinarides, Croatia: A New View.– In: BUSH, D. (ed.): 2007 GSA Denver Annual Meeting and Exhibition: Abstracts with Programs, 146–146.

WU, J., WANG, L., WANG, S., TIAN, R., XUE, C., FENG, W. & LI, Y. (2017): Spatiotemporal variation of groundwater quality in an arid area experiencing long-term paper wastewater irrigation, northwest China.– Environmental Earth Sciences 76, 460. doi: 10.1007/s12665-017-6787-2